A Simple Method for Estimating Intactness of Spinach Leaf Protoplasts by Glycolate Oxidase Assay
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چکیده
منابع مشابه
Effect of oxygen on photosynthesis by spinach leaf protoplasts.
The photosynthetic CO(2) fixation by spinach leaf (Spinacia oleracea L. var. Kyoho) protoplasts was inhibited by substituting an atmosphere of N(2) with one of either air (21% O(2)) or 100% O(2). The inhibitory effect of 100% O(2) was greater than that of air. The mode of inhibition by 100% O(2) and air was competitive with respect to CO(2); Ki(O(2)) value was 0.32 mM at pH 7 and 0.28 mM at pH ...
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Glycolate oxidase, an enzyme that plays an important role in photorespiration in plants, has been purificant from spinach and crystallized in two different crystal forms. Form A which was obtained with tertiary butanol as precipitating agent belongs to space group I 422 with unit cell dimensions a = b = 148.1 A and c = 134.9 A. This form diffracts to high resolution and will be used for further...
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Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expres...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1985
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.78.2.343